Identification of Bacteria in Groundwater Used for Domestic Supply in the Southeast San Joaquin Valley, California, 2014

U.S. Geological Survey (USGS) | June 22nd, 2021

Groundwater is an important source of drinking water in California. Water-borne diseases caused by microbial contamination are a growing concern. The MI test, a membrane

Groundwater is an important source of drinking water in California. Water-borne diseases caused by microbial contamination are a growing concern. The MI test, a membrane filtration method for the chromogenic-/-fluorogenic detection of total coliforms and Escherichia coli, was used for samples collected January to April 2014 from 42 domestic wells in the southeastern San Joaquin Valley. The wells were sampled as part of the Groundwater Ambient Monitoring and Assessment Program Priority Basin Project (GAMA-PBP), a cooperative study between the U.S. Geological Survey and the California State Water Resources Control Board. Polymerase chain reaction analysis and sequencing of deoxyribonucleic acid (DNA) were used for 34 target and nontarget colonies that grew on the MI media from samples collected from 13 of the domestic wells to identify what genera of bacteria could exist in groundwater used by domestic wells. Gene sequences obtained using the Sanger method were entered into the basic local alignment search tool (BLAST) database, and 17 genera of bacteria were identified. Of these, 13 genera contain species that are human pathogens or opportunistic human pathogens. All the genera that include human pathogens are naturally present in soil, plants, or water; one of the pathogens also can be found in fecal matter. Six of the human pathogens were from non-target colony growth on the MI media. Target and non-target microbial growth on MI media are indicators of the possible presence of pathogenic bacteria even if the bacteria naturally are from soil rather than from a fecal source.

Identification of Bacteria in Groundwater Used for Domestic Supply in the Southeast San Joaquin Valley, California, 2014

U.S. Geological Survey (USGS) | June 22nd, 2021

Groundwater is an important source of drinking water in California. Water-borne diseases caused by microbial contamination are a growing concern. The MI test, a membrane

Groundwater is an important source of drinking water in California. Water-borne diseases caused by microbial contamination are a growing concern. The MI test, a membrane filtration method for the chromogenic / fluorogenic detection of total coliforms and Escherichia coli, was used for samples collected January to April 2014 from 42 domestic wells in the southeastern San Joaquin Valley. The wells were sampled as part of the Groundwater Ambient Monitoring and Assessment Program Priority Basin Project (GAMA-PBP), a cooperative study between the U.S. Geological Survey and the California State Water Resources Control Board. Polymerase chain reaction analysis and sequencing of deoxyribonucleic acid (DNA) were used for 34 target and nontarget colonies that grew on the MI media from samples collected from 13 of the domestic wells to identify what genera of bacteria could exist in groundwater used by domestic wells. Gene sequences obtained using the Sanger method were entered into the basic local alignment search tool (BLAST) database, and 17 genera of bacteria were identified. Of these, 13 genera contain species that are human pathogens or opportunistic human pathogens. All the genera that include human pathogens are naturally present in soil, plants, or water; one of the pathogens also can be found in fecal matter. Six of the human pathogens were from non-target colony growth on the MI media. Target and non-target microbial growth on MI media are indicators of the possible presence of pathogenic bacteria even if the bacteria naturally are from soil rather than from a fecal source.

Identification of fresh submarine groundwater off the coast of San Diego, USA, using electromagnetic methods

Hydrogeology Journal (Springer) | March 1st, 2022

Climate change has a pronounced effect on water resources in many semiarid climates, causing populated areas such as San Diego County (USA), to become more vulnerable to

Climate change has a pronounced effect on water resources in many semiarid climates, causing populated areas such as San Diego County (USA), to become more vulnerable to water shortages in the coming decades. To prepare for decreased water supply, San Diego County is adopting policies to decrease water use and to develop additional local sources of water. One new local source of freshwater is produced by a desalination facility that purifies brackish groundwater from the coastal San Diego Formation. This formation has been studied extensively onshore, but little is known about the geology or groundwater quality offshore in the adjacent continental shelf. Because most groundwater systems are interconnected and complex, further analysis is needed to identify offshore geology, possible sequestration of freshwater in the shelf, and potential pathways for saltwater intrusion. This comprehensive understanding is important because seawater intrusion may limit use of the San Diego Formation and longevity of desalination facilities. Controlled-source electromagnetic methods are uniquely suited to detecting offshore groundwater as they are sensitive to changes in pore fluids such as the transition from fresh to brackish groundwater. This paper describes results from surface-towed electromagnetic surveys that mapped the pore-fluid salinity and possible fluid pathways in the continental shelf off the coast of San Diego. The results indicate a considerable volume of fresh-to-brackish groundwater sequestered in the shelf, both in continuous lenses and isolated pockets, that appear influenced by fault systems and shallow stratigraphy.

Identifying and Addressing Contaminant Sources Impacting an Urban Estuary

San Francisco Estuary Institute (SFEI) | July 1st, 2016

Identifying organic contaminants at trespass cannabis grows on federal land in California, USA

Elsevier | September 25th, 2025

Despite the legalization of recreational cannabis in California, USA, illegal cannabis cultivation remains pervasive, partly through the establishment of illegal cult

Despite the legalization of recreational cannabis in California, USA, illegal cannabis cultivation remains pervasive, partly through the establishment of illegal cultivation on public lands (trespass grows). These operations often illegally divert water for irrigation and perform unauthorized applications of chemical fertilizers and pesticides. This work investigates a broad suite of these chemicals, focusing on their persistence in topsoil and presence in water and bed sediment in adjacent streams. Quantitative analyses of pesticides were conducted (183 compounds in water; 176 in topsoil/bed sediment), supplemented by qualitative nontargeted screening at three trespass grows that had no active cultivation for 8 months to 2 years. Targeted multi-residue analysis of topsoil detected the insecticides bifenthrin, cyfluthrin, malathion and imidacloprid (with concentrations up to 38 ng/g dry weight, d.w.), and the fungicides fluopyram, myclobutanil, and triadimefon (concentrations up to 8.1 ng/g d.w.). No pesticides were detected in the companion water or streambed sediment samples from adjacent streams. In addition, no water samples were found to have measurable estrogenic activity. Nontargeted screening uncovered additional pesticides (i.e., spiromesifen, trinexapac) in the topsoil and cannabis-related compounds (i.e., cannabidol, delta9-tetrahydrocannabinol) in both topsoil and streambed sediment suggesting the likelihood of offsite transport of cannabis related compounds. Phthalate plasticizers, rubber-related compounds, pharmaceuticals and personal care product chemicals were detected in topsoil, water, and streambed sediment and may be related to extensive irrigation infrastructure installed at these trespass grows. This work begins to establish a contaminant profile associated with illegal cannabis growing activities, providing a foundation for future research focused on their potential ecological impacts.

Identifying the location and population served by domestic wells in California

Journal of Hydrology: Regional Studies (Elsevier) | November 17th, 2014

Study region: California, USA. Study focus: Identification of groundwater use is an important stepin the regional-scale assessment of groundwater quality. In Califo

Study region: California, USA. Study focus: Identification of groundwater use is an important stepin the regional-scale assessment of groundwater quality. In California, 1990 US Census data indicate that domestic wells provide drinking-water to about 1.2 million people. However, the location of these domestic well users of groundwater is poorly identified because the census tracts can be quite large (up to 20,000 km2). The purposes of this paper are to present methods used for (1) estimating the location of domestic wells, (2) estimating the location of households using domes-tic well water; and (3) identifying where in California groundwater isan important source of domestic drinking supply.New hydrological insights for the region: Aggregating the results indicates that three hydrogeologic provinces contain nearly 80% of all domestic wells and also have the highest density of domestic well-users: Central Valley (31.6%), Sierra Nevada (31.5%), and NorthernCoast Ranges (16.6%). Results were also aggregated into groundwater basins and highland areas, collectively called Groundwater Units(GUs). Twenty-eight of the 938 GUs contain more than 50% of the total population served by domestic wells, 70 GUs contain more than 75%, and 150 GUs contain 90%. The 28 GUs are mostly located in the eastern and southern San Joaquin Valley (11), the Sacramento Valley (7), and the western foothills of the Sierra Nevada province (5). Using the information presented in this research along with other information about domestic-well use, the US Geological Survey has begun sampling high-use GUs for the Shallow Aquifer Assessment component of the Groundwater Ambient Assessment (GAMA) program.

Identifying wastewater chemicals in coastal aerosols

American Association for the Advancement of Science (AAAS) | May 28th, 2025

The Tijuana River, at the US-Mexico border, discharges millions of gallons of wastewater daily—sewage, industrial waste, and runoff—into the Pacific Ocean, making it

The Tijuana River, at the US-Mexico border, discharges millions of gallons of wastewater daily—sewage, industrial waste, and runoff—into the Pacific Ocean, making it the dominant source of coastal pollution in this region. This study examines how such wastewater influences coastal aerosols by tracking spatial gradients from near the border northward. Using benzoylecgonine (a nonvolatile cocaine metabolite) as a sewage tracer, we find that wastewater compounds—including a mixture of illicit drugs, drug metabolites, and chemicals from tires and personal care products—become aerosolized and are detectable in both water and air. Spatial analyses confirm that most measured chemicals concentrate in aerosols near the Tijuana River, potentially exposing local populations to tens of nanograms per hour (e.g., octinoxate and methamphetamine) via inhalation. This airborne pathway highlights a largely overlooked source of atmospheric pollution, emphasizing the need to reassess health risks in coastal regions as global water contamination continues to escalate.

Impacts of ocean acidification on marine organisms: quantifying sensitivities and interaction with warming

Wiley | February 21st, 2013

Ocean acidification represents a threat to marine species worldwide, and forecasting the ecological impacts of acidification is a high priority for science, management, a

Ocean acidification represents a threat to marine species worldwide, and forecasting the ecological impacts of acidification is a high priority for science, management, and policy. As research on the topic expands at an exponential rate, a comprehensive understanding of the variability in organisms' responses and corresponding levels of certainty is necessary to forecast the ecological effects. Here, we perform the most comprehensive meta‐analysis to date by synthesizing the results of 228 studies examining biological responses to ocean acidification. The results reveal decreased survival, calcification, growth, development and abundance in response to acidification when the broad range of marine organisms is pooled together. However, the magnitude of these responses varies among taxonomic groups, suggesting there is some predictable trait‐based variation in sensitivity, despite the investigation of approximately 100 new species in recent research. The results also reveal an enhanced sensitivity of mollusk larvae, but suggest that an enhanced sensitivity of early life history stages is not universal across all taxonomic groups. In addition, the variability in species' responses is enhanced when they are exposed to acidification in multi‐species assemblages, suggesting that it is important to consider indirect effects and exercise caution when forecasting abundance patterns from single‐species laboratory experiments. Furthermore, the results suggest that other factors, such as nutritional status or source population, could cause substantial variation in organisms' responses. Last, the results highlight a trend towards enhanced sensitivity to acidification when taxa are concurrently exposed to elevated seawater temperature.

Impacts of the 2014 severe drought on the Microcystis bloom in San Francisco Estuary

Harmful Algae (Elsevier) | February 17th, 2017

The increased frequency and intensity of drought with climate change may cause an increase in the magnitude and toxicity of freshwater cyanobacteria harmful algal blooms

The increased frequency and intensity of drought with climate change may cause an increase in the magnitude and toxicity of freshwater cyanobacteria harmful algal blooms (CHABs), including Microcystis blooms, in San Francisco Estuary, California. As the fourth driest year on record in San Francisco Estuary, the 2014 drought provided an opportunity to directly test the impact of severe drought on cyanobacteria blooms in SFE. A field sampling program was conducted between July and December 2014 to sample a suite of physical, chemical, and biological variables at 10 stations in the freshwater and brackish reaches of the estuary. The 2014 Microcystis bloom had the highest biomass and toxin concentration, earliest initiation, and the longest duration, since the blooms began in 1999. Median chlorophyll a concentration increased by 9 and 12 times over previous dry and wet years, respectively. Total microcystin concentration also exceeded that in previous dry and wet years by a factor of 11 and 65, respectively. Cell abundance determined by quantitative PCR indicated the bloom contained multiple potentially toxic cyanobacteria species, toxic Microcystis and relatively high total cyanobacteria abundance. The bloom was associated with extreme nutrient concentrations, including a 20-year high in soluble reactive phosphorus concentration and low to below detection levels of ammonium. Stable isotope analysis suggested the bloom varied with both inorganic and organic nutrient concentration, and used ammonium as the primary nitrogen source. Water temperature was a primary controlling factor for the bloom and was positively correlated with the increase in both total and toxic Microcystis abundance. In addition, the early initiation and persistence of warm water temperature coincided with the increased intensity and duration of the Microcystis bloom from the usual 3 to 4 months to 8 months. Long residence time was also a primary factor controlling the magnitude and persistence of the bloom, and was created by a 66% to 85% reduction in both the water inflow and diversion of water for agriculture during the summer. We concluded that severe drought conditions can lead to a significant increase in the abundance of Microcystis and other cyanobacteria, as well as their associated toxins.

Implementing California’s Sustainable Groundwater Management Act (SGMA): Implementing California’s Sustainable Groundwater Management Act (SGMA)

Ostrom Workshop Colloquium Presentation | February 29th, 2016

This research effort is guided by the following overarching research question: What can be learned from existing groundwater management arrangements in Californ

This research effort is guided by the following overarching research question: What can be learned from existing groundwater management arrangements in California — adjudications, special act districts, and select AB 3030 planning processes and county ordi nances — to inform the creation of durable, sustainable groundwater management plans under California’s new Sustainable Groundwater Management Act? The current and planned research described in this presentation is being conducted with a team of researche rs at Stanford University’s Woods Institute on the Environment, Water in the West Program, and Gould Center on Dispute Resolution. Preliminary work is being supported by a grant from Stanford’s Environmental Ventures Program (Janet Martinez, PI).