Chronic, low concentration pesticide exposure alters reproduction and behavior in the intertidal sea anemone, Anthopleura elegantissima

Elsevier | December 10th, 2025

Widespread pesticide and herbicide use paired with frequent transport away from application sites has led to pesticide presence in nearly all terrestrial and aquatic envi

Widespread pesticide and herbicide use paired with frequent transport away from application sites has led to pesticide presence in nearly all terrestrial and aquatic environments globally. Pesticides have unintentional toxic effects on non-target organisms by interfering with cellular processes, behavior, feeding, reproduction, and disrupting endocrine processes. The aggregating anemone, Anthopleura elegantissima, is an important species along the North American Pacific coast due to its symbiotic relationships that contribute to high productivity, and its clonal abundance that structures the rocky intertidal habitat. Commonly used pesticides, atrazine, diuron, and carbendazim were previously detected in coastal waters of Oregon, U.S.A. This study examined the potential effects of these pesticides at environmentally relevant concentrations on reproduction, symbionts, and behavior of A. elegantissima over an eight-week period. Pesticides significantly decreased gonad development in all treatments, having the most significant effect in individual treatments of atrazine (p = 0.003), carbendazim (p = 0.003), and the mixture of all three pesticides (p = 0.008).All pesticide treatments significantly increased cloning behavior compared to the control, suggesting that cloning could be a stress response. Pesticide exposure also significantly increased tentacle retraction movement, suggesting possible metabolic or energy impairments. While other studies have previously found behavioral changes in anemones due to pollutants, our study is the first to document behavioral changes in anemones from pesticide exposure. All three pesticides significantly impacted a non-target marine invertebrate at environmentally relevant concentrations, which underscores the value of studies that focus on effects on marine invertebrates, paired with comprehensive pesticide monitoring in coastal areas.

Clear Lake Watershed and Lake Remediation

University of California, Davis (UC Davis) | December 9th, 2024

The main goal of this joint effort between the University of California Davis, Tahoe Environmental Research Center (UCD-TERC), and the United States Geological Survey (US

The main goal of this joint effort between the University of California Davis, Tahoe Environmental Research Center (UCD-TERC), and the United States Geological Survey (USGS), is to develop the watershed and in-lake monitoring and modeling needed to:  Better understanding of the processes contributing to poor water quality;  Predict watershed and lake responses to changing environmental conditions; and,  Evaluate the impacts and unintended consequences of potential management and restoration activities. 

Climate change-resilient snowpack estimation in the Western United States

Nature Portfolio (Springer Nature) | June 18th, 2024

Coastal wetland deposition of cathode metals from the world’s largest lithium-ion battery fire

Nature Portfolio (Springer Nature) | November 26th, 2025

Comparing and integrating fish surveys in the San Francisco Estuary: Why diverse long-term surveys are important

UnIversity of California, Davis (UC Davis) | June 30th, 2020

Comparing domestic and public-supply groundwater quality in the northern San Joaquin Valley, 2019—California GAMA Priority Basin Project

U.S. Geological Survey (USGS) | June 5th, 2023

Groundwater quality in the Northern San Joaquin Valley region of California was studied as part of California State Water Resources Control Board (SWRCB) Groundwater Ambi

Groundwater quality in the Northern San Joaquin Valley region of California was studied as part of California State Water Resources Control Board (SWRCB) Groundwater Ambient Monitoring and Assessment Program-Priority Basin Project (GAMA-PBP). The GAMA-PBP made a spatially unbiased assessment of the aquifer system used for domestic drinking-water supply in the study region and compared the results to the aquifer system used for public drinking-water supply. These assessments characterized the quality of raw groundwater to evaluate ambient conditions in regional aquifers and not the quality of treated drinking water. The study included two components: (1) a status assessment presenting study results summarizing the status of groundwater quality used for domestic supply in the Northern San Joaquin Valley and (2) a comparative assessment of groundwater resources used for domestic and public drinking-water supply in the study region.

Comparison of the Performance of Different Bioassessment Methods: Similar Evaluations of Biotic Integrity from Separate Programs and Procedures

Journal of the North American Benthological Society (University of Chicago Press) | January 16th, 2006

Regional bioassessment programs of states, various federal agencies, and other governmental and private groups often use different methods to collect and analyz

Regional bioassessment programs of states, various federal agencies, and other governmental and private groups often use different methods to collect and analyze stream invertebrate samples. This lack of uniformity has created concern and confusion over the comparability of disparate sources of data, but few studies have attempted to evaluate differences in performance between methods or to reconcile the results produced from different methods. We conducted concurrent sampling at 40 sites in the eastern Sierra Nevada of California using 3 bioassessment methods to obtain directly comparable data sets. The riffle-based methods (University of California Sierra Nevada Aquatic Research Laboratory [UC-SNARL, Lahontan Water Board], California Stream Bioassessment Protocol, and US Forest Service Region 5) differed at each stage from field sample collection to laboratory processing and data analysis. We used a performance-based methods system to compare precision, uniformity, discrimination, accuracy, and correlations among multimetric Index of Biotic Integrity (IBI) scores and multivariate River Invertebrate Prediction and Classification System (RIVPACS)-type observed/expected (O/E) ratios. Reference and test sites were identified using local and upstream-watershed disturbance criteria, and invertebrate community measures and models were then developed to discriminate between reference and test sites. The more- intensive UC-SNARL method showed slightly, but consistently, greater sensitivity for discriminating impairment than the other 2 methods. The UC-SNARL method produced greater differences between reference- and test-site means relative to lower reference-site standard deviations than the other 2 methods. However, assessment scores were highly correlated among methods and distinguished reference from test sites with similar accuracy among methods despite the slight differences in performance. Our results show that differing bioassessment methods can yield very similar, effective discrimination of impaired biological condition even though they have multiple differences in field and laboratory protocols (mesh size, replication, area sampled, taxonomic resolution, total counts). Moreover, this conclusion did not depend on the approach taken to data analysis because both multimetric IBIs and multivariate RIVPACS-type O/Es were in close agreement. Methodological uniformity is important when coordinating monitoring programs, but our results suggest that data from multiple sources could potentially be used interchangeably and for cross-validation of assessments of stream biological integrity.

Comparisons of Targeted-riffle and Reach-wide Benthic Macroinvertebrate Samples: Implications for Data Sharing in Stream-condition Assessments

Journal of the North American Benthological Society (University of Chicago Press) | January 1st, 2007

Recent comparisons of benthic macroinvertebrate (BMI) sampling protocols have shown that samples collected from different habitat types generally produce consis

Recent comparisons of benthic macroinvertebrate (BMI) sampling protocols have shown that samples collected from different habitat types generally produce consistent stream classifications and assessments. However, these comparisons usually have not included biological endpoints used by monitoring agencies, such as multimetric indices (e.g., benthic index of biotic integrity [B-IBI]) or observed- to-expected (O/E) indices of taxonomic completeness, as target variables, and estimates of method precision are rarely provided. Targeted-riffle (TR) and reach-wide (RW) benthic samples have been collected at thousands of sites across the western USA, but little guidance is available for understanding 1) the extent to which raw data sets can be combined in regional or large-scale analyses, 2) the degree of precision afforded by each method, or 3) the efficacy of cross-application of biological indicators derived from one sample type to the other. To address these issues, we used data from 193 sites in California where the Environmental Monitoring and Assessment Program (EMAP) collected the 2 samples side by side. We also conducted a separate study wherein 3 replicates of each sample type were collected from 15 streams to estimate minimum detectable difference (MDD) as a measure of each method’s precision. Metrics calculated from TR and RW samples showed similar dose–response relationships to stressors gradients and similar raw scoring ranges. Biological indices (B-IBI, O/E0, and O/E50) derived from RW samples were more precise than those derived from TR samples, but precision differences were not substantial. On average, pairwise differences in any index between TR and RW sample types were much less than the MDD associated with either sampling method. We observed a weak but consistent bias toward higher O/E50 scores from TR samples than from RW samples at the highest elevations and in the largest watersheds. Broad-scale condition assessments were nearly identical when B-IBI and O/E0 were used as endpoints, and assessments based on O/E50 were only slightly less similar. Our analyses indicate that raw data sets and biological indicators derived from TR and RW samples may be generally interchangeable when used in ambient biomonitoring programs.

Comprehensive Groundwater Cleanup Strategy for Historical Chromium Discharges from PG&E's Hinkley Compressor Station, San Bernardino County, Final Environmental Impact Report Volume I: Comments on Draft EIR and Responses

California State Water Resources Control Board (SWRCB) | April 20th, 2013

Click here for Volume II

Click here for Volume II

Concentrations for total dissolved solids, arsenic, boron, fluoride, and nitrite-nitrate for wells sampled in the Mojave Water Agency Management Area

U.S. Geological Survey (USGS) | July 1st, 2001