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Estrogen Receptor In Vitro Assay Linkage Studies

Nancy D. Denslow, Kevin Kroll, Alvina Mehinto, Keith Maruya | July 30th, 2018


Our Phase 1 study indicated that it would be possible to link in vitro ER assays to in vivo endpoints (Mehinto et al. 2018). These conclusions were based on several experiments that were performed at the University of Florida and SCCWRP. Our conclusion was that in order to see higher order effects in Menidia, the dose of estrogens in the in vivo assay needed to be above the EC50 point for the in vitro assays. However, the dose response curve for the in vivo endpoints was broad starting at 20 ng E2/L and progressing to 20, 200 and 500 ng/L. Our

Phase 1 experiments did not include the resolution to reduce uncertainty in estimated quantitative in vitro to in vivo linkage factors. While the data allowed us to extrapolate those values, it was decided that the experiment should be repeated another time with more intervening estradiol concentrations, to get a better determination.

A second goal of the study was to measure estrogen equivalencies at 6 locations in San Francisco Bay, testing both the water and sediments, to determine the usefulness of the approach. Results from this study will begin to enable managers to determine whether or not additional cleanup is necessary for treated effluents that are disposed into sensitive estuarine environments.

Keywords

monitoring, Sacramento–San Joaquin Delta, water quality