The effects of kaolin clay on the amphipod Eohaustorius estuarius: Part Two

A number of lines-of-evidence suggest that the amphipod Eohaustorius estuarius has variable tolerance to clay in sediments. In Phase 1 of the current RMP special study, laboratory dose-response experiments were conducted in 2014 with kaolin clay to evaluate whether clay effects varied with amphipod size. The results indicated that smaller amphipods were more tolerant of clay than larger individuals. Average survival was 81%, 79%, and 65% for small, medium and large amphipods, respectively, in concentrations > 50% clay. As part of Phase 2 confirmation studies conducted in 2015-2016, the original kaolin dose-response experiments were repeated. Results of this experiment showed that average amphipod survival was 88%, 63%, and 41% for small, medium and large amphipods, respectively, in concentrations > 50% clay. Standard 96-hour reference toxicant tests with cadmium chloride (CdCl2) were conducted to determine whether there were size-specific differences in response to this metal reference toxicant. The CdCl2 median lethal concentrations (LC50s) for small, medium and large amphipods were 6.78, 5.13, and 4.63 mg/L, respectively. Responses of all three size classes to cadmium were within historic confidence intervals for this reference metal, and were not significantly different from one another based on overlapping confidence intervals. Additional experiments with high clay reference site sediments from San Francisco Bay were conducted to confirm the size related response with field sediments. The results confirm that use of smaller amphipods in routine monitoring of high clay sediments will reduce the influence of this factor on test results.